Original Article

Lung volume reduction surgery in an experimental rat model of emphysema

Cirurgia de redução do volume pulmonar em modelo experimental de enfisema em ratos

Laerte Brasiliense Fusco, Marcelo Heleno Fonseca, Paulo Manuel Pêgo-Fernandes, Rogério Pazetti, Vera Capelozzi, Fabio Biscegli Jatene, Sergio Almeida Oliveira

J Bras Pneumol.2005;31(1):

Background: Lung volume reduction surgery may be a viable treatment alternative for emphysema patients suffering from severe respiratory insufficiency. Objectives: To evaluate functional and morphological aspects of emphysematous rat lungs, prior to and following lung volume reduction surgery. Method: Wistar rats were divided into two experimental groups (papain without surgery and papain with surgery) and three control groups (saline without surgery, saline with surgery and papain without mechanical ventilation). After approximately 40 days of endotracheal instillation of papain or saline solution, animals in the papain with surgery and saline with surgery groups were submitted to bilobectomy of the middle lobes by right thoracotomy along the posterior border of the superior vena cava. After 1 week, the same animals were submitted to a mechanical ventilation study, which involved measurement of lung elasticity and airway resistance. For all of the animals studied, lung tissue was analyzed in order to determine alveolar diameter and the elastic fiber quantity. Results: Morphometric analysis revealed higher mean alveolar diameter in the lungs of all animals exposed to papain as compared to those exposed to saline. Elastic fiber counts in the alveolar septa of animals treated with papain were lower than those of animals receiving saline. In the animals submitted to bilobectomy and papain, lung elasticity was greater than in those receiving papain without surgery and was statistically equal to that seen in animals receiving saline (with or without surgery). Conclusion: In the respiratory systems of animals with pulmonary emphysema submitted to lung volume reduction by bilobectomy, the capacity for elastic recoil returned to values equivalent to those of the control group animals.

Keywords: Key words: Pulmonary emphysema. Papain/drugs efects. Case-control studies. Disease models, animal. Respiratory mechanics/drugs efects. Lung/sugery. Lung/anatomy & histology.

List of reviewers

Relationship of Reviewers volume 46 (1-6) 2020

Relação de revisores do volume 46 (1-6) 2020

J Bras Pneumol.2020;46(6):.

Original Article

Papain-induced experimental pulmonary emphysema model in rats

Modelo experimental de enfisema pulmonar em ratos induzido por papaína

Laerte Brasiliense Fusco, Paulo M. Pêgo-Fernandes, Alexandre Martins Xavier, Rogério Pazetti, Dolores Helena Rodriguez Ferreira Rivero, Vera Luiza Capelozzi, Fábio Biscegli Jatene

J Bras Pneumol.2002;28(1):1-7

Objective: The reproduction of an experimental emphysema model in rats by intratracheal instillation of papain was proposed for the evaluation of lung volume reduction surgeries. Method: The study used orotracheal instillation of papain (20 mg/kg) dissolved in 0.9% saline solution. Forty days after instillation, animals underwent mechanical ventilation. Elastance and resistance of the respiratory system were measured, and the rats were sacrificed by having their lungs removed. The pulmonary tissue of the animals was qualitatively analyzed with hematoxylineosin staining and submitted to morphometric analysis for the measurement of the mean alveolar diameter. The pulmonary tissue was also subjected to resorcin-fuchsin staining for the identification of elastic fibers, which were quantified in alveolar septa by digital imaging. Results: In the animals instilled with papain, the histological analysis of the lungs showed pan-acinar emphysema, with rupture of alveolar septa and hyperdistention. Morphometric analysis showed higher mean values for mean alveolar diameter in the lungs of the animals submitted to papain (149.08 μm and 100.56 μm) as compared to the group receiving saline solution (64.08 μm and 75.90 μm). The quantification of elastic fibers of alveolar septa of papain-treated animals was 70% lower than in the animals receiving saline solution. Mechanic ventilation did not show differences in respiratory system resistance of animals receiving papain or saline solution. Respiratory system elastance was lower in the group receiving papain than in the group receiving saline solution, showing a functional status compatible with pulmonary emphysema, with decreased elasticity of the pulmonary tissue. Conclusion: The reproduction of an experimental model of pan-acinar pulmonary emphysema in rats was achieved by papain instillation through the respiratory tree with functional and morphologic evidences.

Keywords: Pulmonary emphysema. Experimental model. Papain. Rats. Drug instillations.

Letters to the Editor

The use of animals in experimental studies

O uso de animais em estudos experimentais

Sami Karapolat

J Bras Pneumol.2008;34(11):989-

Case Report

Pneumothorax as a complication of lung volume recruitment

Pneumotórax como complicação associada ao recrutamento do volume pulmonar

Erik J.A. Westermann, Maurice Jans, Michael A. Gaytant, John R. Bach, Mike J. Kampelmacher

J Bras Pneumol.2013;39(3):382-386

Index of Issues

Índice remissivo de assuntos do volume 44 (1-6) 2018

J Bras Pneumol.2018;44(6):533-534

Index of Authors

Índice remissivo de autores do volume 44 (1-6) 2018

J Bras Pneumol.2018;44(6):535-538

List of reviewers

Relação de revisores do volume 44 (1-6) 2018

J Bras Pneumol.2018;44(6):539-542

List of reviewers for volume 48 (1-6) 2022

Relação de revisores do volume 48 (1-6) 2022

Jornal Brasileiro de Pneumologia

J Bras Pneumol.2022;48(6):rev2022

List of reviewers for volume 49 (1-6) 2023

Relação de revisores do volume 49 (1-6) 2023

Jornal Brasileiro de Pneumologia

J Bras Pneumol.2023;49(6):.

Original Article

Pulmonary reperfusion injury by aortic occlusion: experimental model in rats

Lesão pulmonar de reperfusão por oclusão da aorta abdominal: modelo experimental em ratos

Bruno do Valle Pinheiro, Cândida Maria Moreira Horta, Bruno Guedes Baldi, Leonardo de Lucca Schiavon, Ângela Maria Gollner, Júlio César Abreu de Oliveira

J Bras Pneumol.2000;26(4):163-168

Brief Communication

Experimental diabetes mellitus: oxidative stress and changes in lung structure

Estresse oxidativo e alterações estruturais pulmonares no diabetes mellitus experimental

Luiz Alberto Forgiarini Junior, Nélson Alexandre Kretzmann, Marilene Porawski, Alexandre Simões Dias, Norma Anair Possa Marroni

J Bras Pneumol.2009;35(8):788-791

Original Article

Experimental models for assessment of pulmonary alterations in hepatopulmonary syndrome

Modelos experimentais para avaliação das alterações pulmonares na síndrome hepatopulmonar

Rafael Vercelino1, Juliana Tieppo, Luiz Albeto Forgiarini Junior, Alexandre Simões Dias, Claudio Augusto Marroni, Norma Possa Marroni

J Bras Pneumol.2008;34(7):453-460

Analysis of pulmonary mechanics in an experimental model of sepsis

Análise da mecânica pulmonar em modelo experimental de sepse

Rodrigo Storck Carvalho, André Gustavo Magalhãer de Pinho, Ana Paula Alves de Andrade, César Augusto Melo e Silva, Carlos Eduardo Gaio, Paulo Tavares

J Bras Pneumol.2006;32(4):316-321

Comparison of two experimental models of pulmonary hypertension

Comparação de dois modelos experimentais de hipertensão pulmonar

Igor Bastos Polônio, Milena Marques Pagliarelli Acencio, Rogério Pazetti, Francine Maria de Almeida, Mauro Canzian, Bárbara Soares da Silva, Karina Aparecida Bonifácio Pereira, Rogério de Souza

J Bras Pneumol.2012;38(4):452-460

Brief Communication

Ex vivo experimental model: split lung block technique

Modelo experimental ex vivo com bloco pulmonar dividido

Alessandro Wasum Mariani, Israel Lopes de Medeiros, Paulo Manuel Pêgo-Fernandes, Flávio Guimarães Fernandes, Fernando do Valle Unterpertinger, Lucas Matos Fernandes, Mauro Canzian, Fábio Biscegli Jatene

J Bras Pneumol.2011;37(6):791-795

Original Article

Pre-treatment with dexamethasone attenuates experimental ventilator-induced lung injury

Pré-tratamento com dexametasona atenua a lesão pulmonar induzida por ventilação mecânica em modelo experimental

Fernando Fonseca dos Reis1,2, Maycon de Moura Reboredo1,2, Leda Marília Fonseca Lucinda1,2, Aydra Mendes Almeida Bianchi1,2, Maria Aparecida Esteves Rabelo1, Lídia Maria Carneiro da Fonseca1,2, Júlio César Abreu de Oliveira1, Bruno Valle Pinheiro1,2

J Bras Pneumol.2016;42(3):166-173

Low tidal volume mechanical ventilation and oxidative stress in healthy mouse lungs

Ventilação mecânica com baixo volume corrente e estresse oxidativo em pulmões saudáveis de camundongos

Karla Maria Pereira Pires, Adriana Correa Melo, Manuella Lanzetti, Natália Vasconcelos Casquilho, Walter Araújo Zin, Luís Cristóvão Porto, Samuel Santos Valença

J Bras Pneumol.2012;38(1):98-104

Editorial

Forced expiratory volume in one second and bronchodilator response in chronic obstructive pulmonary disease - a needless ritual?

Volume expiratório forçado no primeiro segundo e resposta a broncodilatador em doença pulmonar obstrutiva crônica - um ritual inútil?

Carlos Alberto de Castro Pereira

J Bras Pneumol.2005;31(5):4-6

Original Article

Radiological profile of the ideal candidate for lung volume reduction surgery to treat emphysema: a systematic review

Perfil radiológico do candidato ideal à cirurgia redutora de volume pulmonar no enfisema: uma revisão sistemática

Paula Antonia Ugalde Figueroa, Martha Silvia Martinez-Silveira, Eduardo Ponte, Aquiles Camelier, Jorge L. Pereira-silva

J Bras Pneumol.2005;31(3):197-204

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